DPABH-25127 detects endogenous levels of EIF2S1 only when phosphorylated at Serine 49.Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.Store at -20°C. Stable for 12 months at -20°C *   http://www.creative-diagnostics.com/Anti-EIF2S1-PAb-197944-489.htm